I figured I could put my screwups in the lab to good use with a new feature, "Lisa does the experiment". All you non-lab people out there, don't worry. My screwups typically extend beyond the lab world, and they will also be included.
We'll start with a double-whammy today!
A. Western blotting mayhem
You know how you're suppossed to soak your membrane in methanol before you try to transfer proteins onto it? Did you ever wonder what would happen if you... say... forgot? Well, I did the experiment, so I can tell you! The proteins do not transfer! Hurrah.
In the interest of full disclosure, I didn't actually COMPLETE this experiment. I realized after I had assembled 4 blot sandwiches (yes I know non-science people, that sounds dumb. blot sandwiches.) that I hadn't done the methanol thing and I had the foresight to ask my PI what would happen. It turns out that he did the experiment in the past, all the way through to its sad finish.
B. Password-typing crazy
This morning I was sitting here and my screen saver came on. We have to have our screen savers password protected, so I had to input my password to get back into my computer. But it wasn't working. In fact, about 2/3 of my keyboard wasn't working. I thought I had broken my poor old powerbook and I very nearly called my wonderful mac support guy. But no! I had done the following experiment: What happens when you accidentally turn on 'number lock'? I'll tell you! only the keys that have numbers on them work!