When I was a grad student, I used to think it was strange that one of the postdocs in our lab didn't wear gloves when she did tissue culture. I guess it is strange, but now I do it too. I guess I'm too lazy to wear gloves. My new PI doesn't wear gloves for anything, even things I wouldn't dream of doing without gloves. And he is the one who taught me. Also, gloves are MURDER on my nails, which have enough problems as it is. So I just ethanol my hands real good while I'm doing TC.
Remember how I said that I had ripped a big strip of skin off of my pinky the other day? Well, I didn't. Until I ethanoled my hands. HOLY CRAP.
On the plus side, the wound is clean now. I don't know that my pain receptors will ever be the same, but we're clean.